Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)‐4‐hydroxy‐3‐methylbut‐2‐enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe–4S] protein

FEBS Letters

Tritsch, Denis³  Rohmer, Michel³  Frapart, Yves¹  Boronat, Albert⁴  Rodrı́guez-Concepción, Manuel⁴  Tse Sum Bui, Bernadette²  Estrabot, Ana Garcia⁴  Marquet, Andrée²  Wolff, Murielle³  Seemann, Myriam³ 

[1] Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, Université René Descartes – Paris V, UMR CNRS 8601, 45 rue des Saints-Pères, 75270 Paris Cedex 06, France;Laboratoire de Chimie Organique Biologique, Université Paris VI, UMR CNRS 7613, 4 place Jussieu, 75252 Paris Cedex 05, France;Université Louis Pasteur, UMR CNRS 7123, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg Cedex, France;Departament de Bioquı́mica i Biologia Molecular, Facultat de Quı́mica, Universitat de Barcelona, Martı́ i Franquès 1, 08028 Barcelona, Spain

关键词: Dimethylallyl diphosphate;    Isopentenyl diphosphate;    Isoprenoid;    LytB;    2-C-Methyl-D-erythritol 4-phosphate pathway;    (E)-4-Hydroxy-3-methylbut-2-enyl diphosphate;    DAF;    5-deazaflavin (10-methyl-5-deazaisoalloxazine);    DMAPP;    dimethylallyl diphosphate;    EPR;    electron paramagnetic resonance;    HMBPP;    (E)-4-hydroxy-3-methylbut-2-enyl diphosphate;    IPP;    isopentenyl diphosphate;    ME;    2-C-methyl-D-erythritol;    MEP;    2-C-methyl-D-erythritol 4-phosphate;   

DOI  :  10.1016/S0014-5793(03)00317-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The last enzyme (LytB) of the methylerythritol phosphate pathway for isoprenoid biosynthesis catalyzes the reduction of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate into isopentenyl diphosphate and dimethylallyl diphosphate. This enzyme possesses a dioxygen-sensitive [4Fe–4S] cluster. This prosthetic group was characterized in the Escherichia coli enzyme by UV/visible and electron paramagnetic resonance spectroscopy after reconstitution of the purified protein. Enzymatic activity required the presence of a reducing system such as flavodoxin/flavodoxin reductase/reduced nicotinamide adenine dinucleotide phosphate or the photoreduced deazaflavin radical.

【 授权许可】

Unknown