A Synechococcus leopoliensis SAUG 1402‐1 operon harboring the 1‐deoxyxylulose 5‐phosphate synthase gene and two additional open reading frames is functionally involved in the dimethylallyl diphosphate synthesis

FEBS Letters

Heuser, Thomas¹  Miller, Barbara¹  Zimmer, Wolfgang¹ 

[1] Fraunhofer Institut für Atmosphärische Umweltforschung, Kreuzeckbahnstr. 19, D-82467 Garmisch-Partenkirchen, Germany

关键词: 1-Deoxyxylulose 5-phosphate synthase gene;    Dimethylallyl diphosphate;    Isopentenyl diphosphate;    Isoprenoid biosynthesis;    dxs;   

DOI  :  10.1016/S0014-5793(99)01397-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Experiments have been performed to prove the existence and the functionality of the novel mevalonate independent 1-deoxyxylulose 5-phosphate isoprenoid biosynthesis pathway in cyanobacteria. For this purpose, a segment of the 1-deoxyxylulose 5-phosphate synthase gene (dxs) was amplified from Synechococcus leopoliensis SAUG 1402-1 DNA via PCR using oligonucleotides for conserved regions of dxs. Subsequent hybridization screening of a genomic cosmid library of S. leopoliensis with this segment has led to the identification of an 18.7 kbp segment of the S. leopoliensis genome on which a dxs homologous gene and two adjacent open reading frames organized in one operon could be localized by DNA sequencing. The three genes of the operon were separately expressed in Escherichia coli, proving that the identified cyanobacterial dxs is functionally involved in the formation of dimethylallyl diphosphate, one basic intermediate of isoprenoid biosynthesis.

【 授权许可】

Unknown