Isopentenyl diphosphate isomerase deficiency in Synechocystis sp. strain PCC6803

FEBS Letters

Cunningham, Francis X.¹  Ershov, Yuri¹  Gantt, R.Raymond¹  Gantt, Elisabeth¹ 

[1] Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA

关键词: Dimethylallyl diphosphate;    Isoprenoid biosynthesis;    Escherichia coli;    Haematococcus;    Synechococcus PCC7942;    Synechocystis PCC6803;   

DOI  :  10.1016/S0014-5793(00)01516-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isopentenyl diphosphate isomerase (IPP isomerase) in many organisms and in plastids is central to isoprenoid synthesis and involves the conversion between IPP and dimethylallyl diphosphate (DMAPP). It is shown that Synechocystis PCC6803 is deficient in IPP isomerase activity, consistent with the absence in its genome of an obvious homologue for the enzyme. Incorporation of [1-¹⁴C]IPP in cell extracts, primarily into C₂₀, occurs only upon priming with DMAPP in Synechocystis PCC6803 and in Synechococcus PCC7942. Isoprenoid synthesis in these cyanobacteria does not appear to involve interconversion of IPP and DMAPP, raising the possibility that they are not within the plastid evolutionary lineage.

【 授权许可】

Unknown