Genetic evidence of branching in the isoprenoid pathway for the production of isopentenyl diphosphate and dimethylallyl diphosphate in Escherichia coli

FEBS Letters

Campos, Narciso²  Hoeffler, Jean-François¹  Maldonado, Carlos²  Rohmer, Michel¹  Boronat, Albert²  Rodrı́guez-Concepción, Manuel²  Grosdemange-Billiard, Catherine¹  Marı́a Lois, Luisa² 

[1] Université Louis Pasteur/CNRS, Institut Le Bel, 4 rue Blaise Pascal, 67070 Strasbourg Cedex, France;Departament de Bioquı́mica i Biologia Molecular, Facultat de Quı́mica, Universitat de Barcelona, Martı́ i Franquès 1-7, 08028 Barcelona, Spain

关键词: 1-Deoxy-D-xylulose 5-phosphate reductoisomerase;    Dimethylallyl diphosphate;    Isopentenyl diphosphate;    Isopentenyl diphosphate isomerase;    Isoprenoid biosynthesis;    Mevalonic acid;   

DOI  :  10.1016/S0014-5793(00)01552-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An alternative mevalonate-independent pathway for isoprenoid biosynthesis has been recently discovered in eubacteria (including Escherichia coli) and plant plastids, although it is not fully elucidated yet. In this work, E. coli cells were engineered to utilize exogenously provided mevalonate and used to demonstrate by a genetic approach that branching of the endogenous pathway results in separate synthesis of the isoprenoid building units isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). In addition, the IPP isomerase encoded by the idi gene was shown to be functional in vivo and to represent the only possibility for interconverting IPP and DMAPP in this bacterium.

【 授权许可】

Unknown