Expression of prokaryotic 1‐deoxy‐d‐xylulose‐5‐phosphatases in Escherichia coli increases carotenoid and ubiquinone biosynthesis

FEBS Letters

Bramley, P.M.¹  Harker, M.¹ 

[1]School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK

关键词: Isoprenoid;    Isopentenyl diphosphate;    Carotenoid;    Bioengineering;    Ubiquinone;    Biosynthesis;   

DOI  :  10.1016/S0014-5793(99)00360-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Isopentenyl diphosphate (IPP) acts as the common, five-carbon building block in the biosynthesis of all isoprenoids. The first reaction of IPP biosynthesis in Escherichia coli is the formation of 1-deoxy-d-xylulose-5-phosphate, catalysed by 1-deoxy-d-xylulose-5-phosphate synthase (DXPS). E. coli engineered to produce lycopene, was transformed with dxps genes cloned from Bacillus subtilis and Synechocystis sp. 6803. Increases in lycopene levels were observed in strains expressing exogenous DXPS compared to controls. The recombinant strains also exhibited elevated levels of ubiquinone-8. These increases corresponded with enhanced DXP synthase activity in the recombinant E. coli strains.

【 授权许可】

Unknown