Novel type Arabidopsis thaliana H+‐PPase is localized to the Golgi apparatus

FEBS Letters

Nakata, Mami¹  Mitsuda, Nobutaka²  Sato, Masa H.³  Enami, Kazuhiko³  Takeyasu, Kunio¹ 

[1] Graduate School of Biostudies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501, Japan;Graduate School of Human and Environmental Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501, Japan;Department of Natural Environmental Sciences, Faculty of Integrated Human Studies, Kyoto University, Yoshida Nihonmatsu-cho, Sakyo-ku, Kyoto 606-8501, Japan

关键词: AVP2/AVPL1 gene;    Golgi apparatus;    Green fluorescent protein;    Brefeldin A;    H+-PPase;    Arabidopsis thaliana;    GFP;    green fluorescent protein;    MS;    Murashige–Skoog;   

DOI  :  10.1016/S0014-5793(00)02400-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vacuolar H⁺-PPase, a membrane bound proton-translocating pyrophosphatase found in various species including plants, some protozoan and prokaryotes, has been demonstrated to be localized to the vacuolar membrane in plants. Using a GUS reporter system and a green fluorescent protein (GFP) fusion protein, we investigated the tissue distribution and the subcellular localization, respectively, of a novel type H⁺-PPase encoded by AVP2/AVPL1 identified in the Arabidopsis thaliana genome. We showed that AVP2/AVPL1 is highly expressed at the trichome and the filament of stamen. Furthermore, the fluorescence of GFP-tagged AVP2/AVPL1 showed small dot-like structures that were observed throughout the cytoplasm of various Arabidopsis cells under a fluorescent microscope. The distribution of this dot-like fluorescent pattern was apparently affected by a treatment with brefeldin A. Moreover, we demonstrated that most dot-like fluorescent structures colocalized with a Golgi resident protein. These findings suggest that this novel type H⁺-PPase resides on the Golgi apparatus rather than the vacuolar membrane.

【 授权许可】

Unknown