NSF is required for the brefeldin A‐promoted disassembly of the Golgi apparatus

FEBS Letters

Tani, Katsuko¹  Hatsuzawa, Kiyotaka¹  Furuno, Akiko¹  Yamamoto, Akitsugu²  Tagaya, Mitsuo¹  Fukunaga, Takuya¹ 

[1] School of Life Science, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192-0392, Japan;Department of Physiology, Kansai Medical University, Moriguchi, Osaka 570-0074, Japan

关键词: Brefeldin A;    Golgi apparatus;    Microinjection;    Retrograde transport;    N-Ethylmaleimide-sensitive factor;    BFA;    brefeldin A;    CHO;    Chinese hamster ovary;    COPI;    Golgi coat proteins;    ER;    endoplasmic reticulum;    mAb 2C8;    monoclonal antibody named 2C8;    man II;    mannosidase II;    NRK;    normal rat kidney;    NSF;    N-ethylmaleimide-sensitive factor;    SNAP;    soluble NSF attachment protein;    SNARE;    SNAP receptor;   

DOI  :  10.1016/S0014-5793(98)01081-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N-Ethylmaleimide-sensitive factor (NSF) is required for multiple pathways of vesicle-mediated protein transport. Microinjection of a monoclonal anti-NSF antibody almost completely blocked brefeldin A-promoted Golgi disassembly without affecting the rapid release of β-COP, a subunit of the Golgi coat proteins (COPI), from the Golgi apparatus. Similar results were obtained using a dominant-negative NSF which is known to compete with endogenous NSF. The present results suggest that an NSF-mediated step is present in the brefeldin A-promoted disassembly of the Golgi apparatus.

【 授权许可】

Unknown