The biochemical profile of rat testicular tissue as measured by magic angle spinning 1H NMR spectroscopy

FEBS Letters

Shore, R.F.¹  Nicholson, J.K.²  Griffin, J.L.²  Lindon, J.C.²  Troke, J.²  Walker, L.A.¹ 

[1] NERC Centre for Ecology and Hydrology, Monks Wood, Huntingdon, Cambridgeshire PE28 2LS, UK;Biological Chemistry, Biomedical Sciences Division, The Sir Alexander Fleming Building, Imperial College of Science, Technology and Medicine, South Kensington SW7 2AZ, UK

关键词: Magic angle spinning 1H NMR;    Testis;    Creatine;    Diffusion weighted spectroscopy;   

DOI  :  10.1016/S0014-5793(00)02307-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The testis is the principal organ of male fertility, responsible for the production of spermatozoa and their maturation into sperm. However, the underlying biochemistry of the testis is relatively understudied. The fluidic and homogeneous nature of the testis makes it an ideal organ for high resolution magic angle spinning (MAS) ¹H NMR spectroscopy. In this study we have catalogued the low molecular weight metabolites. The testis contains large amounts of creatine, of which a substantial proportion was shown to be extracellular using bipolar gradients to measure apparent diffusion coefficients. The tissue also contained relatively high amounts of uridine.

【 授权许可】

Unknown