Identification of creatine as a cofactor of thiamin‐diphosphate kinase

FEBS Letters

Egi, Yoshiko¹  Shikata, Hiroo¹  Koyama, Shin-ya¹  Yamada, Kazuo¹  Kawasaki, Takashi¹ 

[1] Department of Biochemistry, Hiroshima University School of Medicine, 1-2-3 Kasumi, Hiroshima 734, Japan

关键词: Creatine;    Cofactor;    Thiamin-diphosphate kinase;    Thiamin triphosphate;   

DOI  :  10.1016/0014-5793(86)80578-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thiamin-diphosphate (TDP) kinase which catalyzes thiamin triphosphate formation from TDP requires a low-molecular-mass cofactor in addition to ATP and Mg²⁺. The cofactor was isolated in a crystalline form from pig skeletal muscle and identified as creatine by proton NMR, mass spectrometry, infrared spectrometry and elemental analysis. The isolated cofactor and authentic creatine supported the same activity of partially purified TDP kinase at identical molar concentrations. Neither creatine phosphate nor creatinine showed activity as a cofactor. This is the first report showing evidence of the existence of a creatine-dependent enzyme.

【 授权许可】

Unknown