CuI‐semiquinone radical species in plant copper‐amine oxidases

FEBS Letters

Bellelli, Andrea³  Padiglia, Alessandra²  Pedersen, Jens Z.⁴  Medda, Rosaria²  Finazzi Agrò, Alessandro¹  Floris, Giovanni² 

[1] Department of Experimental Medicine, University of Rome ‘Tor Vergata’, Rome, Italy;Department of Biochemistry and Human Physiology, University of Cagliari, Monserrato, 09042 Cagliari, Italy;CNR Center of Molecular Biology, Department of Biochemical Sciences, University of Rome ‘La Sapienza’, Rome, Italy;Department of Chemistry, Odense University, Odense, Denmark

关键词: Lentil;    Amine oxidase;    Copper;    Cofactor;    6-Hydroxydopa;    Lens esculenta;    Cu-AO;    copper-amine oxidase;    GABA;    γ-aminobutyric acid;    LSAO;    lentil seedling amine oxidase;    p-DABA;    p-(dimethylamino)-benzylamine;    p-DAMBA;    p-((dimethylamino)methyl)-benzylamine;    PSAO;    pea seedling amine oxidase;    TPQ;    6-hydroxydopa (2;    4;    5-trihydroxyphenethylamine) quinone;   

DOI  :  10.1016/S0014-5793(99)00675-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intermediate Cu^I-semiquinone radical species in the catalytic mechanism of copper-amine oxidase from Lens esculenta and Pisum sativum seedlings has been studied by optical, Raman resonance and ESR spectroscopies and by stopped-flow and temperature-jump measurements. Treatment of highly purified enzyme preparations with good, poor or suicide substrates, under anaerobic and aerobic conditions, at different pH values and temperatures, makes it possible to generate, detect and characterize this free radical intermediate.

【 授权许可】

Unknown