Oxidation products of polyamines induce mitochondrial uncoupling and cytochrome c release

FEBS Letters

Maccarrone, M¹  Falciglia, K¹  Bari, M¹  Battista, N¹  Di Rienzo, M¹  Finazzi Agrò, A¹ 

[1]Department of Experimental Medicine and Biochemical Sciences, University of Rome ‘Tor Vergata’, Via di Tor Vergata 135, I-00133 Rome, Italy

关键词: Amine oxidase;    Apoptosis;    Cytochrome c;    Hydrogen peroxide;    Mitochondrial potential;    Polyamine;    AO;    amine oxidase;    BSA;    bovine serum albumin;    CCCP;    m-chlorophenylhydrazone;    ΔΨ;    membrane potential;    ELISA;    enzyme-linked immunosorbent assay;    GAM-AP;    goat anti-mouse alkaline phosphatase conjugates;    JC-1;    5;    5′;    6;    6′-tetrachloro-1;    1′;    3;    3′-tetraethyl-benzimidazol-carbocyanine iodide;    MIB;    mitochondria incubation buffer;    MPT;    mitochondrial permeability transition;    PBS;    phosphate-buffered saline;    PCD;    programmed cell death;   

DOI  :  10.1016/S0014-5793(01)02949-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spermine is shown to uncouple isolated mitochondria and to trigger the selective release of cytochrome c. Pargyline, an inhibitor of amine oxidase (AO), fully prevented these effects of spermine, which instead were potentiated by exogenous AO. Hydrogen peroxide, an oxidation product of spermine, mimicked the effects of spermine on mitochondria, while the addition of catalase prevented them. Spermidine and putrescine also caused mitochondrial uncoupling and triggered cytochrome c release, with a potency which correlated with the substrate preference of mitochondrial AO. Pargyline protected human lymphoma U937 cells against UVB-induced apoptosis, by reducing AO activity, mitochondrial uncoupling and cytochrome c release.

【 授权许可】

Unknown