Cytochrome c release and caspase activation in hydrogen peroxide‐ and tributyltin‐induced apoptosis

FEBS Letters

Kimland, Monica¹  Jones, Dean P.¹  Orrenius, Sten¹  Hampton, Mark B.¹  Stridh, Hélène¹ 

[1]Institute of Environmental Medicine, Division of Toxicology, Karolinska Institutet, Box 210, S-171 77 Stockholm, Sweden

关键词: Cytochrome c;    Caspase;    Hydrogen peroxide;    Tributyltin;    Mitochondrion;    Membrane potential;   

DOI  :  10.1016/S0014-5793(98)00630-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ability of H₂O₂ and tributyltin (TBT) to trigger pro-caspase activation via export of cytochrome c from mitochondria to the cytoplasm was investigated. Treatment of Jurkat T lymphocytes with H₂O₂ resulted in the appearance of cytochrome c in the cytosol within 2 h. This was at least 1 h before caspase activation was observed. TBT caused cytochrome c release already after 5 min, followed by caspase activation within 1 h. Measurement of mitochondrial membrane potential (ΔΨ_m) showed that both H₂O₂ and TBT dissipated ΔΨ_m, but with different time courses. TBT caused a concomitant loss of ΔΨ_m and release of cytochrome c, whereas cytochrome c release and caspase activation preceded any apparent ΔΨ_m loss in H₂O₂-treated cells. Thus, our results suggest that different mechanisms are involved in triggering cytochrome c release with these apoptosis-inducing agents.

【 授权许可】

Unknown