A putative nuclear receptor coactivator (TMF/ARA160) associates with hbrm/hSNF2α and BRG‐1/hSNF2β and localizes in the Golgi apparatus

FEBS Letters

Kato, Hiroyuki¹  Mori, Katsuhiro¹ 

[1] Faculty of Pharmaceutical Sciences, Hokkaido University, N12 W6, Sapporo 060-0812, Japan

关键词: Transcription;    SWI/SNF;    Nuclear receptor;    Cofactor;    Golgi apparatus;   

DOI  :  10.1016/S0014-5793(02)02803-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An ATP-dependent chromatin remodeling factor, SNF/SWI complex, acts as a coactivator for numerous transcriptional factors. One of the best-documented examples is nuclear receptors, although the molecular mechanism for this coactivation has not been sufficiently elucidated. Here we show that hbrm/hSNF2α and BRG-1/hSNF2β, the ATPase subunits of the human SNF/SWI complexes, specifically associate in vitro and in vivo with TATA element modulatory factor (TMF)/ARA160, which has been described as a binding protein to and coactivator for the androgen receptor. This interaction requires highly conserved N-terminal regions of hbrm/hSNF2α and BRG-1/hSNF2β and a C-terminal region of TMF/ARA160. Immunofluorescence and Western blot studies revealed that the TMF isoforms differentially localize in the Golgi apparatus and the nucleus.

【 授权许可】

Unknown