The amino acid sequences of human and pig l‐arginine:glycine amidinotransferase

FEBS Letters

Mann, Karlheinz¹  Humm, Andreas¹  Huber, Robert¹ 

[1] Max-Planck-Institut für Biochemie, Am Klopferspitz, D-82152 Martinsried, Germany

关键词: l-Arginine: glycine amidinotransferase;    Amino acid sequence;    Creatine;    APMSF;    4-aminophenyl methanesulfonyl fluoride;    DTT;    dithiothreitol;    PMSF;    phenylmethanesulfonyl fluoride;    TLCK;    losyl lysine chloromethyl ketone;    TPCK;    tosyl phenylalanine chloromethyl ketone;   

DOI  :  10.1016/0014-5793(94)80394-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated and sequenced the l-arginine:glycine amidinotransferase of pig kidney mitochondria. Due to endogenous proteolysis, the purified molecules showed some heterogeneity at the N terminus. The longest form recovered had 386 amino acids. Part of the pig amidinotransferase sequence information was used to isolate cDNA clones coding for the human enzyme. The deduced amino acid of the human amidinotransferase was 37 amino acids longer due to the presence of a signal sequence. The mature proteins were 94% identical to each other and 36% identical to the sequences of bacterial l-argininednosamine phosphate amidinotransferases.

【 授权许可】

Unknown