Elevated expression of membrane type 1 metalloproteinase (MT1‐MMP) in reactive astrocytes following neurodegeneration in mouse central nervous system

FEBS Letters

Budde, Petra²  Staege, Martin Sebastian²  Bartsch, Jörg W.¹  Schlomann, Uwe¹  Frey, Jürgen²  Rathke-Hartlieb, Silvia¹  Ewert, Stefan²  Jockusch, Harald¹ 

[1] Developmental Biology and Molecular Pathology, W7, University of Bielefeld, D-33501 Bielefeld, Germany;Faculty of Chemistry, Biochemistry II, University of Bielefeld, P.O. Box 100131, D-33501 Bielefeld, Germany

关键词: Neurodegeneration;    Wobbler mouse;    Astrocyte;    Membrane type matrix metalloproteinase 1;    Tissue inhibitor of metalloproteinase;    Tumor necrosis factor α;    CNS;    central nervous system;    ECM;    extracellular matrix;    EGF;    epidermal growth factor;    GFAP;    glial fibrillary acidic protein;    MDF;    muscle deficient;    MMP;    matrix metalloproteinase;    MND2;    motoneuron disease 2;    MT-MMP;    membrane type MMP;    TGFα;    transforming growth factor α;    TIMP;    tissue inhibitor of metalloproteinase;    TNF-α;    tumor necrosis factor α;    WR;    wobbler;    WT;    wild-type;   

DOI  :  10.1016/S0014-5793(00)02011-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Reactive astrocytes occurring in response to neurodegeneration are thought to play an important role in neuronal regeneration by upregulating the expression of extracellular matrix (ECM) components as well as the ECM degrading metalloproteinases (MMPs). We examined the mRNA levels and cellular distribution of membrane type matrix metalloproteinase 1 (MT1-MMP) and tissue inhibitors 1–4 of MMPs (TIMPs) in brain stem and spinal cord of wobbler (WR) mutant mice affected by progressive neurodegeneration and astrogliosis. MT1-MMP, TIMP-1 and TIMP-3 mRNA levels were elevated, whereas TIMP-2 and TIMP-4 expression was not affected. MT1-MMP was expressed in reactive astrocytes of WR. In primary astrocyte cultures, MT1-MMP mRNA was upregulated by exogeneous tumor necrosis factor α. Increased plasma membrane and secreted MMP activities were found in primary WR astrocytes.

【 授权许可】

Unknown