Protein synthesis is required for stabilization of hsp70 mRNA upon exposure to both hydrostatic pressurization and elevated temperature

FEBS Letters

Eriksson, John E.²  Holmberg, Carina I.²  Lammi, Mikko J.¹  Kaarniranta, Kai¹  Sistonen, Lea²  Helminen, Heikki J.¹ 

[1] Department of Anatomy, University of Kuopio, P.O. Box 1627, 70211 Kuopio, Finland;Turku Centre for Biotechnology, University of Turku, Åbo Akademi University, P.O. Box 123, 20521 Turku, Finland

关键词: Heat shock protein;    mRNA stabilization;    Hydrostatic pressure;    ActD;    actinomycin D;    CHX;    cycloheximide;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    Hsc;    heat shock protein cognate;    HSF1;    heat shock transcription factor 1;    Hsp;    heat shock protein;    HP;    hydrostatic pressure;   

DOI  :  10.1016/S0014-5793(00)01693-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently described that in chondrocytic cells high hydrostatic pressure (HP) causes a heat shock response via mRNA stabilization without a transcriptional activation of the hsp70 gene. In this study, we investigated whether this exceptional regulatory mechanism occurs more generally in different types of cells. Indeed, hsp70 mRNA and protein accumulated in HeLa, HaCat and MG-63 cells under 30 MPa HP, without DNA-binding of heat shock transcription factor 1 (HSF1) to the heat shock element of the hsp70 gene or formation of nuclear HSF1 granules, revealing a lack of transcriptional activation. Moreover, we observed that protein synthesis is needed for mRNA stabilization. Thus, high HP offers a model to study the mechanisms of hsp70 mRNA stabilization without HSF1-mediated induction of the heat shock gene response.

【 授权许可】

Unknown