Stabilization of mRNA in an Escherichia coli cell‐free translation system

FEBS Letters

Yoshizawa, Satoko¹  Hirao, Ichiro¹  Miura, Kin-ichiro¹ 

[1] Department of Industrial Chemistry, Faculty of Engineering, The University of Tokyo, Hongo, Tokyo 113, Japan

关键词: Stable DNA hairpin;    In vitro translation;    mRNA stabilization;   

DOI  :  10.1016/0014-5793(93)80101-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It became clear that mRNA can be stabilized in a cell-free translation system of Escherichia coli by hybridization with a small DNA fragment at its 3' terminus. The stability increased when a small DNA fragment containing a stable hairpin structure with a GAAA loop was used. The enhancement of stabilization was brought about because the hairpin structure is resistant towards the nucleases contained in the translation system. The hairpin structure is effective by stabilizing the added DNA fragment itself towards the nucleases.

【 授权许可】

Unknown