Detection of lipofuscin‐like fluorophore in oxidized human low‐density lipoprotein

FEBS Letters

Itakura, Koichi³  Uchida, Koji²  Yamada, Satoshi⁴  Osawa, Toshihiko²  Oya-Ito, Tomoko²  Kobayashi, Makio⁵  Shibata, Noriyuki⁵  Toyokuni, Shinya¹ 

[1] Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto 606, Japan;Laboratory of Food and Biodynamics, Nagoya University Graduate School of Bioagricultural Sciences, Nagoya 464-8601, Japan;Faculty of Education, Aichi University of Education, Kariya 448, Japan;Tsukuba Research Laboratory, NOF Co., Tsukuba 300-2635, Japan;Department of Pathology, Tokyo Women's Medical University, Tokyo 162-8666, Japan

关键词: Lipid peroxidation;    4-Hydroxy-2-nonenal;    Fluorescent chromophore;    Low-density lipoprotein;    Atherosclerosis;    HIDP;    hydroxyiminodihydropyrrole;    HNE;    4-hydroxy-2-nonenal;    ONE;    4-oxo-2-nonenal;    LDL;    low-density lipoprotein;    apoB;    apolipoprotein B-100;    BSA;    bovine serum albumin;    PBS;    phosphate-buffered saline;    ELISA;    enzyme-linked immunosorbent assay;    KLH;    keyhole limpet hemocyanin;   

DOI  :  10.1016/S0014-5793(00)01539-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has recently been shown that the lipid peroxidation product 4-hydroxy-2-nonenal (HNE) forms a fluorescent hydroxyiminodihydropyrrole derivative with the ϵ-amino group of lysine residue. In this study, we raised a monoclonal antibody (mAb2C12) directed to the fluorophore–protein conjugate and found that the antibody was specific to the chromophore structure of the compound. Immunohistochemical analysis of atherosclerotic lesions from the human aorta showed that the fluorophore was indeed present in the lesions, in which intense positivity was primarily associated with macrophage-derived foam cells and thickening of the neointima of the arterial walls. Antigenic materials were also detected in the oxidatively modified low-density lipoprotein (LDL) with Cu²⁺ and in the oxidatively modified bovine serum albumin with an iron/linoleic acid autoxidation system, indicating that the HNE, which originated from the peroxidation of polyunsaturated fatty acids, could be a potential source of the fluorescent chromophore in oxidized LDL.

【 授权许可】

Unknown