Activation of a capacitative Ca2+ entry pathway by store depletion in cultured hippocampal neurones

FEBS Letters

Bouron, Alexandre¹ 

[1] Department of Pharmacology, University of Bern, Friedbuehlstrasse 49, Bern, Switzerland

关键词: Hippocampus;    Capacitative calcium entry;    Store-operated channel;   

DOI  :  10.1016/S0014-5793(00)01340-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Intracellular Ca²⁺ ([Ca²⁺]_i) changes were measured in cell bodies of cultured rat hippocampal neurones with the fluorescent indicator Fluo-3. In the absence of external Ca²⁺, the cholinergic agonist carbachol (200 μM) and the sarco-endoplasmic reticulum Ca²⁺ pump inhibitor thapsigargin (0.4 μM) both transiently elevated [Ca²⁺]_i. A subsequent addition of Ca²⁺ into the bathing medium caused a second [Ca²⁺]_i change which was blocked by lanthanum (50 μM). Taken together, these experiments indicate that stores depletion can activate a capacitative Ca²⁺ entry pathway in cultured hippocampal neurones and further demonstrate the existence of such a Ca²⁺ entry in excitable cells.

【 授权许可】

Unknown