Concomitant and hormonally regulated expression of trp genes in bovine aortic endothelial cells

FEBS Letters

Chang, Albert S.²  Chang, Sharon M.¹  Garcia, Reynaldo L.³  Schilling, William P.³ 

[1]Department of Medicine, MetroHealth Medical Center, 2500 MetroHealth Drive, Cleveland, OH 44109, USA

[2]Rammelkamp Center for Research, MetroHealth Medical Center, 2500 MetroHealth Drive, Cleveland, OH 44109, USA

[3]Department of Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH, USA

关键词: Capacitative calcium entry;    Fura-2;    Steroid hormone;    Semi-quantitative PCR;    Vascular endothelium;    Thapsigargin;   

DOI  :  10.1016/S0014-5793(97)01155-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recent findings have suggested that the vertebrate trp family of channel proteins is the structural basis for Ca²⁺ influx through the capacitative calcium entry (CCE) pathway. We have discerned, in bovine aortic endothelial cells, the concomitant expression of four such vertebrate genes: trp-1 (two splice variants), trp-3, trp-4 and trp-5. Exogenous hormones rendered dynamic effects on the transcript levels of these genes. Most notably, β-estradiol significantly down-regulated trp-4 while trans-retinoic acid dramatically up-regulated trp-5; yet these hormones rendered little change in CCE. These findings suggest that the extent of a given trp channel's participation in CCE is not reflected in alterations of its transcript level.

【 授权许可】

Unknown