2‐APB inhibits volume‐regulated anion channels independently from intracellular calcium signaling modulation

FEBS Letters

Lemonnier, L.¹  Shuba, Y.¹  Prevarskaya, N.¹  Mazurier, J.¹  Skryma, R.¹ 

[1] Laboratoire de Physiologie Cellulaire, INSERM EMI 0228, Bâtiment SN3, USTL, 59655 Villeneuve d’Ascq, France

关键词: Volume-regulated anion channel;    Store-operated channel;    2-Aminoethoxydiphenyl borate;    Calcium;    Capacitative entry;   

DOI  :  10.1016/S0014-5793(03)01387-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has previously been suggested that volume-regulated anion channels (VRACs) and store-operated channels (SOCs) interact with each other according to their expected colocalization in the plasma membrane of LNCaP cells. In order to study interactions between these two channels, we used 2-aminoethoxydiphenyl borate (2-APB) as a regular SOC inhibitor. Surprisingly 2-APB reduced VRAC activity in a dose-dependent manner (IC₅₀=122.8 μM), but not 2,2-diphenyltetrahydrofuran (a structural analog of 2-APB). This effect was also present in keratinocytes. We conclude that 2-APB is an inhibitor of the VRAC family, and is also a potent tool to study the SOC–VRAC interaction in LNCaP cells.

【 授权许可】

Unknown