Acyl and alkyl chain length of GPI‐anchors is critical for raft association in vitro

FEBS Letters

Simons, Kai¹  Benting, Jürgen¹  Rietveld, Anton¹  Ansorge, Iris² 

[1] European Molecular Biology Laboratory (EMBL), Cell Biology and Biophysics Programme, Postfach 102209, Meyerhofstrasse 1, 69117 Heidelberg, Germany;Zentrum für Molekulare Biologie, Universität Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany

关键词: GPI-anchor;    Fatty acid;    Lipid raft;    Chol;    cholesterol;    ESI-MS;    electrospray ionization mass spectrometry;    OG;    N-octyl-β-D-glyco-pyranoside;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    PI;    phosphatidylinositol;    PLAP;    placental alkaline phosphatase;    Sm;    sphingomyelin;    Gal-Cer;    O-β-D-galactosyl(1→1)ceramide;    TX-100;    Triton X-100;    VSG;    variant surface glycoprotein;    VSV-PLAP;    VSV-G epitope-tagged PLAP;   

DOI  :  10.1016/S0014-5793(99)01501-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We determined the acyl and alkyl chain composition of GPI-anchors isolated from MDCK and Fischer rat thyroid (FRT) cells. Both cell lines synthesize GPI-anchors containing C16/C18 or C18/C18 saturated acyl and alkyl chains. The GPI-anchored placental alkaline phosphatase (PLAP) expressed in both cells is raft-associated and PLAP purified from FRT cells is raft-associated in vitro when reconstituted into liposomes containing raft lipids. In contrast, the GPI-anchored variant surface glycoprotein from Trypanosoma brucei which contains C14 acyl and alkyl chains shows no significant raft association after reconstitution in vitro. These data indicate that the acyl and alkyl chain composition of GPI-anchors determines raft association.

【 授权许可】

Unknown