Identification of two cell cycle regulated genes affecting the β1,3‐glucan content of cell walls in Saccharomyces cerevisiae

FEBS Letters

Brekelmans, S.S.C.²  Oehlen, L.J.W.M.¹  Klis, F.M.²  Ram, A.F.J.² 

[1]The Rockefeller University, New York, NY 10021, USA

[2]Institute for Molecular Cell Biology, University of Amsterdam, BioCentrum Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands

关键词: GAS1;    GGP1;    Calcofluor white;    Cell wall;    Cell cycle arrest;    β-Glucan;    CFW;    Calcofluor white;    SCB;    SW14;    6-dependent cell cycle box;    MCB;    MluI cell cycle box;    MES;    2-[N-morpholino]-ethanesulfonic acid;    GPI-anchor;    glycosylphosphatidylinositol anchor;    ConA;    concanavalin A;    FITC;    fluorescein isothiocyanate;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(94)01418-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Calcofluor white-hypersensitive mutants cwh52 and cwh53 are severely reduced in β1,3-glucan. CWH52 was equivalent to GAS1. CWH53 represented a new gene, located on the right arm of chromosone XII, and predicted to encode a 215 kDa protein with multiple transmembrane domains. The transcription of CWH53 was cell cycle-dependent and, similar to GAS1/CWH52, increased in late G₁, indicating that the formation of β-glucan is cell cycle-regulated. Further, in some mutant alleles of both gas1/cwh52 and cwh53 lethal concentrations of Calcofluor induced growth arrest at a specific phase of the cell cycle.

【 授权许可】

Unknown