The proposed straw separation system developed in the research project harvests the large internode sections of the straw which has the greater potential as a feedstock for lignocellulosic ethanol production while leaving the chaff and nodes in the field. This strategy ensures sustainable agriculture by preventing the depletion of soil minerals, and it restores organic matter to the soil in amounts and particle sizes that accommodate farmers needs to keep tillage and fertilizer costs low. A ton of these nutrient-rich plant tissues contains as much as $10.55 worth of fertilizer (economic and energy benefits), in terms of nitrogen, phosphorus, potassium, and other nutrients provided to the soil when incorporated by tillage instead of being burned. Biomass conversion to fermentable sugars for the purpose of producing fuels, chemicals, and other industrial products is well understood. Most bioenergy strategies rely on low-cost fermentable sugars for sustainability and economic viability in the marketplace. Exploitation of the whole cropspecifically, wheat straw or other plant material currently regarded as residue or waste is a practical approach for obtaining a reliable and low-cost source of sugars. However, industrial-scale production of sugars from wheat straw, while technically feasible, is plagued by obstacles related to capital costs, energy consumption, waste streams, production logistics, and the quality of the biomass feedstock.