A novel function for a ubiquitous plant enzyme pectin methylesterase: the host‐cell receptor for the tobacco mosaic virus movement protein

FEBS Letters

Kalkkinen, N.¹  Saarinen, J.¹  Mäkinen, K.¹  Merits, A.¹  Saarma, M.¹  Atabekov, J.G.³  Dorokhov, Yu.L.²  Frolova, O.Yu.³ 

[1] Institute of Biotechnology, Viikki Biocenter, P.O. Box 56 (Viikinkaari 9), Fin-00014 University of Helsinki, Helsinki, Finland;A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia;Department of Virology, Moscow State University, Moscow 119899, Russia

关键词: Tobamovirus;    Movement protein;    Cell-to-cell movement;    Tobacco;    Pectin methylesterase;    Receptor;    BOBA;    blot overlay binding assay;    CW;    cell wall;    MP;    movement protein;    PME;    pectin methylesterase;    PD;    plasmodesmata;    TMV;    tobacco mosaic virus;   

DOI  :  10.1016/S0014-5793(99)01447-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plant virus-encoded movement proteins promote viral spread between plant cells via plasmodesmata. The movement is assumed to require a plasmodesmata targeting signal to interact with still unidentified host factors presumably located on plasmodesmata and cell walls. The present work indicates that a ubiquitous cell wall-associated plant enzyme pectin methylesterase of Nicotiana tabacum L. specifically binds to the movement protein encoded by tobacco mosaic virus. We also show that pectin methylesterase is an RNA binding protein. These data suggest that pectin methylesterase is a host cell receptor involved in cell-to-cell movement of tobacco mosaic virus.

【 授权许可】

Unknown