Expression and biochemical analyses of the recombinant potato virus X 25K movement protein

FEBS Letters

Fedorkin, O.N.²  Korpela, T.¹  Morozov, S.Yu.²  Atabekov, J.G.²  Kalinina, N.O.²  Samuilova, O.V.²  Maiss, E.³ 

[1] Joint Finnish-Russian Biological Laboratory, University of Turku, SFF-20500 Turku, Finland;Department of Virology of Moscow State University, Moscow 119899, Russian Federation;Institute for Biochemistry and Plant Virology, University of Hannover, Herrenhaeuser Str. 2, D-30419 Hannover, Germany

关键词: Plant RNA virus;    Movement protein;    ATPase;    RNA binding;   

DOI  :  10.1016/S0014-5793(96)01138-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 25K movement protein (MP) of potato virus X (PVX) is encoded by the 5′-proximal gene of three overlapping MP genes forming a ‘triple gene block’. The PVX 25K MP (putative NTPase-helicase) has been synthesized in Escherichia coli as a recombinant containing a six-histidine tag at the amino terminus. The His-tagged 25K protein was purified in a one-column Ni-chelate affinity chromatography procedure. In the absence of any other viral factors, this protein had obvious Mg²⁺-dependent ATPase activity, which was stimulated slightly (1.7–1.9-fold) by various polynucleotides. Like other viral proteins possessing ATPase-helicase motifs and many plant viral movement proteins, the PVX 25K MP was able to bind nucleic acids in vitro. The RNA binding activity of the 25K MP was pronounced only at very low salt concentrations and was independent of its ATPase activity.

【 授权许可】

Unknown