First evidence showing that Pepper vein yellows virus P4 protein is a movement protein

BMC Microbiology

article

Li, Sangsang¹  Liu, Yong¹  Su, Xianyan³  Luo, Xiangwen²  Zhang, Yu¹  Zhang, Deyong¹  Du, Jiao²  Zhang, Zhanhong²  OuYang, Xian²  Zhang, Songbai¹ 

[1] Longping Branch, Hunan University;Key Laboratory of Pest Management of Horticultural Crop of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural Science;Plant Protection Institute of Anhui Academy of Agricultural Science

关键词: Pepper vein yellows virus;    P4 protein;    Plasmodesmata;    Movement protein;    Cell-to-cell movement;   

DOI  :  10.1186/s12866-020-01758-y

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Plant viruses move through plasmodesmata (PD) to infect new cells. To overcome the PD barrier, plant viruses have developed specific protein(s) to guide their genomic RNAs or DNAs to path through the PD. In the present study, we analyzed the function of Pepper vein yellows virus P4 protein. Our bioinformatic analysis using five commonly used algorithms showed that the P4 protein contains an transmembrane domain, encompassing the amino acid residue 117–138. The subcellular localization of P4 protein was found to target PD and form small punctates near walls. The P4 deletion mutant or the substitution mutant constructed by overlap PCR lost their function to produce punctates near the walls inside the fluorescent loci. The P4-YFP fusion was found to move from cell to cell in infiltrated leaves, and P4 could complement Cucumber mosaic virus movement protein deficiency mutant to move between cells. Taking together, we consider that the P4 protein is a movement protein of Pepper vein yellows virus.

【 授权许可】

CC BY|CC0