Indomethacin‐induced G1/S phase arrest of the plant cell cycle

FEBS Letters

Witters, Erwin¹  Inzé, Dirk²  Roef, Luc¹  Van Onckelen, Harry¹  Reichheld, Jean-Philippe²  Ehsan, Hashimul¹  Van Bockstaele, Dirk³ 

[1] Laboratorium voor Plantenbiochemie en -fysiologie, Department of Biology, Universiteit Antwerpen (UIA), Universiteitsplein 1, B-2610 Antwerp, Belgium;Laboratorium voor Genetica, Department of Plant Genetics, Flanders Inter University Institute for Biotechnology, Universiteit Gent, B-9000 Ghent, Belgium;Laboratory of Experimental Hematology, Universiteit Antwerpen UIA-UZA, Wilrijkstraat 10, B-2650 Edegem, Belgium

关键词: Indomethacin;    Propyzamide;    Tobacco bright yellow 2 cell;    Cell cycle;    Synchronization;    Cyclic AMP;    cAMP;    adenosine 3′;    5′-cyclic monophosphate;    TBY-2;    tobacco bright yellow 2;    CDK;    cyclin dependent kinase;   

DOI  :  10.1016/S0014-5793(99)01152-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In animal systems, indomethacin inhibits cAMP production via a prostaglandin-adenylyl cyclase pathway. To examine the possibility that a similar mechanism occurs in plants, the effect of indomethacin on the cell cycle of a tobacco bright yellow 2 (TBY-2) cell suspension was studied. Application of indomethacin during mitosis did not interfere with the M/G1 progression in synchronized BY-2 cells but it inhibited cAMP production at the beginning of the G1 phase and arrested the cell cycle progression at G1/S. These observations are discussed in relation to the putative involvement of cAMP biosynthesis in the cell cycle progression in TBY-2 cells.

【 授权许可】

Unknown