The fas locus of the phytopathogen Rhodococcus fascians affects mitosis of tobacco BY‐2 cells

FEBS Letters

Temmerman, Wim¹  Inzé, Dirk¹  Mironov, Vladimir¹  Simón-Mateo, Carmen¹  Holsters, Marcelle¹  Ritsema, Tita¹  Van Montagu, Marc¹  Goethals, Koen¹ 

[1] Vakgroep Moleculaire Genetica and Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: Aphidicolin;    Cytokinin;    Mitotic index;    Propyzamide;    Virulence;    BAP;    6-benzyl aminopurine;    BY-2;    Bright Yellow-2;    CDK;    cyclin-dependent kinase;    2;    4-D;    2;    4-dichlorophenoxyacetic acid;    DAPI;    2′;    6-diamidino-2-phenylindole;    DMSO;    dimethylsulfoxide;    ipt;    isopentenyl transferase;    %MI;    mitotic index;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/S0014-5793(01)02248-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of Rhodococcus fascians, the causal agent of leafy gall disease, on the mitotic behavior of synchronized tobacco Bright Yellow-2 (BY-2) cells was investigated. Incubation of aphidicolin-synchronized BY-2 cells with R. fascians cells specifically resulted in a broader mitotic index peak, an effect that was linked to an intact and expressed fas virulence locus. The obtained results pointed towards an effect of R. fascians on the prophase of mitosis. The relevance of these results to the virulence of the bacterium is discussed.

【 授权许可】

Unknown