Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin

Journal of Biological Research

Emmanuel Panteris¹  Barbara-Evelin Diannelidis² 

[1] Aristotle University of Thessaloniki;National and Kapodistrian University of Athens

关键词: Aphidicolin;    Arabidopsis thaliana;    Cell division;    Katanin;    Microtubule orientation;    Microtubule severing;   

DOI  :  10.1186/s40709-018-0082-6

学科分类：生物科学（综合）

来源: Springer

【 摘 要 】

BackgroundTransverse cortical microtubule orientation, critical for anisotropic cell expansion, is established in the meristematic root zone. Intending to elucidate the possible prerequisites for this establishment and factors that are involved, microtubule organization was studied in roots of Arabidopsis thaliana, wild-type and the p60-katanin mutants fra2, ktn1-2 and lue1. Transverse cortical microtubule orientation in the meristematic root zone has proven to persist under several regimes inhibiting root elongation. This persistence was attributed to the constant moderate elongation of meristematic cells, prior to mitotic division. Therefore, A. thaliana wild-type seedlings were treated with aphidicolin, in order to prevent mitosis and inhibit premitotic cell elongation.

【 授权许可】

Free