COMPARING MOLECULAR PLASTICITY RESPONSES OF ARABIDOPSIS THALIANA RILS TO COLD STRATIFICATION

Adaptation - Biology;Biodiversity;Species diversity;Germination;Arabidopsis thaliana

Yakub, Mohamed I.Dorn, Lisa ;

University of Wisconsin

关键词: Adaptation - Biology;    Biodiversity;    Species diversity;    Germination;    Arabidopsis thaliana;   

Others  :  https://minds.wisconsin.edu/bitstream/handle/1793/49185/YakubThesis_%28With_TP%29.pdf?sequence=1&isAllowed=y

瑞士|英语

来源: University of Wisconsin

【 摘 要 】

Phenotypic plasticity is the ability of genetically identical organisms to producediffering phenotypes in response to environmental heterogeneity. While there are twocentral models for the genetic basis of phenotypic plasticity few studies have identifiedthe genetic and molecular basis of plasticity. I used three recombinant inbred lines(RILs), of Arabidopsis thaliana generated by crossing two natural genotypes, andobtaining progeny after recombination, for a global gene expression study. Of the threeRILs, one bolted (transition to reproductive tissue) later if the seeds experienced a coldstratificationperiod relative to the seeds that did not experience this cold-treatment(sensitive-positive), one bolted earlier if the seeds experienced the cold-stratificationrelative to the non-cold treated seeds (sensitive-negative), and one did not change when itbolted regardless of the cold stratification of seeds (homeostatic). I found different sets ofgenes differentially expressed in the three RILs suggesting that the response to the sameregime of cold-stratification is via different pathways. The homeostatic genotypeincreased transcription of protein metabolism genes in response to cold stratification;whereas the plastic genotypes differentially expressed genes involved in stress response.This cold-stratification which is a common dormancy breaking cue for these plants mayalso be perceived as a stress, as the plants differentially expressed stress genes. Thus bothgene expression and perception of environment may be involved in plastic responses toenvironmental heterogeneity.