Effect of indomethacin on cell cycle dependent cyclic AMP fluxes in tobacco BY‐2 cells

FEBS Letters

Witters, Erwin¹  Inzé, Dirk³  Lardon, Filip²  Roef, Luc¹  Van Onckelen, Harry¹  Reichheld, Jean-Philippe³  Van Montagu, Marc³  Ehsan, Hashimul¹  Van Bockstaele, Dirk² 

[1] Laboratorium voor Plantenbiochemie en -fysiologie, Dept. of Biology, Universiteit Antwerpen (UIA), Universiteitsplein 1, B-2610 Wilrijk, Belgium;Laboratory of Experimental Hematology, Universiteit Antwerpen UIA-UZA, Wilrijkstraat 10, B-2650 Edegem, Belgium;Laboratorium voor Genetica, Department of Genetics, Flanders University Institute for Biotechnology, Universiteit Gent, B-9000 Ghent, Belgium

关键词: Cell cycle;    Adenosine 3′;    5′-cyclic monophosphate;    Indomethacin;    Mass spectrometry;    Synchronisation;    Tobacco BY-2 cell;    cAMP;    adenosine 3′;    5′-cyclic monophosphate;    BY-2;    bright yellow 2;    LC;    liquid chromatography;    MS;    mass spectrometry;    RP;    reversed phase;    MRM;    multiple reaction monitoring;    MeOH;    methanol;    HAc;    acetic acid;    Aϕ;    aphidicolin;    PZ;    propyzamide;    IM;    indomethacin;   

DOI  :  10.1016/S0014-5793(97)01610-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The evolution of adenosine 3′,5′-cyclic monophosphate (cAMP) levels was investigated in synchronised tobacco BY-2 cells by virtue of a method based on immunoaffinity purification and analysis on electrospray tandem mass spectrometry. A transient peak in cAMP content was observed during the S and G₁ phases of the cell cycle. Application of the prostaglandin inhibiting drug indomethacin at early S phase resulted in the loss of the cAMP peak in S phase and inhibited mitotic division. This inhibition of cAMP accumulation suggests the presence of a prostaglandin-dependent adenylyl cyclase activity, analogous to animal cyclases. A potential role for cAMP during the plant cell cycle is postulated.

【 授权许可】

Unknown