Heptameric association of light‐harvesting complex II trimers in partially solubilized photosystem II membranes

FEBS Letters

van Roon, Henny²  Dekker, Jan P.²  Boekem, Egbert J.¹ 

[1] Groningen Biotechnology and Biomolecular Sciences Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands;Dept. of Physics and Astronomy, Institute of Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands

关键词: Photosystem II;    Light-harvesting complex II;    Heptamer;    Electron microscopy;    C2S2;    PSII-LHCII supercomplex consisting of a dimeric PSII core complex and two strongly bound LHCII complexes;    a-DM;    n-dodecyl-α;    D-maltoside;    EM;    electron microscopy;    LHCII;    light-harvesting complex II;    PSII;    photosystem II;   

DOI  :  10.1016/S0014-5793(99)00442-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report a structural characterization by electron microscopy and image analysis of a supramolecular complex consisting of seven trimeric light-harvesting complex II proteins. The complex was readily observed in partially-solubilized Tris-washed photosystem II membranes from spinach but was also found to occur, with a low frequency, in oxygen-evolving photosystem II membranes. The structure reveals six peripheral trimers with the same rotational orientation and a central trimer with the opposite orientation. We conclude that the heptamer represents a naturally occurring aggregation state of part of the light-harvesting complex II trimers in the thylakoid membranes.

【 授权许可】

Unknown