Specific association of photosystem II and light‐harvesting complex II in partially solubilized photosystem II membranes

FEBS Letters

Boekema, Egbert J²  Dekker, Jan P¹  van Roon, Henny¹ 

[1] Department of Physics and Astronomy, Institute of Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, Netherlands;Department of Biophysical Chemistry, BIOSON Research Centre, University of Groningen, Nijenborgh 4, 9747 AG Groningen, Netherlands

关键词: Photosystem II;    Photosynthesis;    Electron microscopy;    Chl a;    chlorophyll a;    Chl b;    chlorophyll b;    α-DM;    n-dodecyl-α;    d-maltoside;    β-DM;    n-dodecyl-β;    d-maltoside;    EM;    electron microscopy;    HPLC;    high-performance liquid chromatography;    LHC II;    light-harvesting complex II;    PS II;    photosystem II;   

DOI  :  10.1016/S0014-5793(98)00147-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study, we report the structural characterization of photosystem II complexes obtained from partially solubilized photosystem II membranes. Direct observation by electron microscopy, within a few minutes after a mild disruption of the membranes with the detergent n-dodecyl-α,d-maltoside, revealed the presence of several large supramolecular complexes. Images of these complexes were subjected to multivariate statistical analysis and classification procedures, resolving a new complex consisting of the previously characterized dimeric supercomplex of photosystem II and light-harvesting complex II [Boekema et al., Proc. Natl. Acad. Sci. USA 92 (1995) 175–179] and two additional, symmetrically organized protein masses each containing a second type of trimeric light-harvesting II complex. We conclude that large and labile integral membrane proteins, such as photosystem II, can be quickly structurally characterized without extensive purification.

【 授权许可】

Unknown