Isolation and properties of PS II membrane fragments depleted of the non heme iron center

FEBS Letters

Andréasson, L.-E.¹  Kurreck, J.³  Garbers, A.²  Renger, G.³  Reifarth, F.³  Parak, F.² 

[1] Department of Biochemistry and Biophysics, Chalmers University of Technology and Göteborg University, Göteborg S-412-96, Sweden;Fakultät für Physik, E 17, Technische Universität München, 85747 Garching, Germany;Max-Volmer-Institut für Biophysikalische und Physikalische Chemie, Technische Universität Berlin, Straβe des 17. Juni 135, D-10623 Berlin, Germany

关键词: Photosystem II;    Non heme iron;    Cytochrome b559;    Mössbauer spectroscopy;    Flash induced fluorescence;    Chl a;    chlorophyll a;    CoRh;    cobalt rhodium;    Cyt b559;    cytochrome b559;    DCMU;    3(3;    4-dichlorophenyl)-1;    1-dimethylurea;    LED;    light emitting diode;    MES;    morpholinoethane sulfonic acid;    P680;    photoactive chlorophyll of PS II;    Pheo;    pheophytin;    QA;    bound plastoquinone-9 acceptor of PS II;    QB;    plastoquinone-9 in QB-site of PS II;    PQH2;    plastoquinol-9;    PS II;    photosystem II;    Yz;    redox active tyrosine between P680 and the water oxidizing complex;   

DOI  :  10.1016/0014-5793(96)00061-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The functional properties and the content of non heme iron and cytochrome b559 were investigated by measuring flash induced transient changes of the relative fluorescence quantum yield and applying Mössbauer spectroscopy. It was found that untreated PS II membrane fragments contain a heterogeneous population of two types of non heme iron centers and about 2 cytochrome b559 per PS II. Twofold treatment of these samples with a recently described ‘iron depletion’ procedure (MacMillan, F., Lendzian, F., Renger, G. and Lubitz, W. (1995) Biochemistry 34, 3144–8156) leads to a complete loss (below the detection limit of Mössbauer spectroscopy) of the non heme iron center while more than 50% of the PS II complexes retain the functional integrity for light induced formation of the ‘stable’ radical pair Y_Z ^OX P680Pheo Q_A ^−.. This sample type deprived of virtually all non heme iron in PS II provides a most suitable material for magnetic resonance studies that require an elimination of the interaction between Fe²⁺ and nearby radicals.

【 授权许可】

Unknown