Detection of calcium binding by photosystem II polypeptides immobilised onto nitrocellulose membrane

FEBS Letters

Webber, Andrew N.¹  Gray, John C.¹ 

[1] Department of Botany, University of Cambridge, Downing Street, Cambridge CB2 3EA, England

关键词: Calcium-binding polypeptide;    Light-harvesting complex II;    Photosystem II;    N-terminal sequence;    DCCD;    N;    N′-dicyclohexylcarbodiimide;    LHC;    light-harvesting complex;    OGP;    1-O-n-octyl-β-D-glucopyranoside;    PVDF;    polyvinylidene difluoride;   

DOI  :  10.1016/0014-5793(89)80020-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photosystem II calcium-binding polypeptides have been detected by their ability to selectively bind ⁴⁵Ca when immobilised onto nitrocellulose membrane following SDS-polyacrylamide gel electrophoresis. Two calcium-binding polypeptides of 26 kDa and 24 kDa are shown to be components of LHCII. The 24 kDa polypeptide was further characterised by N-terminal amino acid sequence analysis and shown to be the product of a type II cab gene. A third polypeptide of 33 kDa bound calcium more weakly and was not positively identified.

【 授权许可】

Unknown