N‐terminal sequence determination and secondary structure analysis of extrinsic membrane proteins in the water‐splitting complex of spinach

FEBS Letters

Vater, Joachim²  Salnikow, Johann²  Jansson, Christer¹ 

[1] Department of Biochemistry, University of Lund, S-22100 Lund, Sweden;Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, Franklinstraße 29, D-1000 Berlin 10, Germany

关键词: Photosystem II;    CD;    Photosynthesis;    Water;    splitting;    Membrane protein;    N-terminal sequence;   

DOI  :  10.1016/0014-5793(86)80748-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The N-terminal sequences of the 16 and 23 kDa extrinsic membrane proteins in photosystem II of spinach have been determined by solid-phase sequencing. The sequence of the 23 kDa protein has been corroborated by a small 3 kDa CNBr fragment confirming partial N-terminal processing by a protease of unknown nature. A secondary structure analysis of the 23 kDa protein by circular dichroism has been performed indicating a β-structure content of 61 % with only 14% α-helix conformation.

【 授权许可】

Unknown