Size, shape and mass of the oxygen‐evolving photosystem II complex from the thermophilic cyanobacterium Synechococcus sp

FEBS Letters

Boekema, E.J.¹  Dekker, J.P.²  Rögner, M.²  Witt, H.T.² 

[1] Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, 1000 Berlin 33, FRG;Max- Volmer-Institut für Biophysikalische und Physikalische Chemie, Technische Universität Berlin, Strasse des 17. Juni 135, 1000 Berlin 12, FRG

关键词: Photosystem II;    Electron microscopy;    Structure;    (Synechococcus);    APC;    allophycocyanin;    β-DM;    β-dodecyl-D-maltoside;    Chl;    chlorophyll;    HPLC;    high performance liquid chromatography;    PC;    phycocyanin;    PS;    photosystem;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(87)81217-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two different, highly active O₂-evolving photosystem II complexes were purified from the cyanobacterium Synechococcus sp. in the presence of the non-ionic detergent β-dodecyl-D-maltoside. Both complexes are homogeneous and have molecular masses of approx. 300 and 500 kDa, respectively. By electron microscopy it was found that both complexes have the shape of an elliptical disk, with a thickness of about 6.5 nm and top view dimensions of 10.5 × 15.5 nm for the 300 kDa particle and 18.5 × 15 nm for the 500 kDa particle. It is concluded that the particles represent monomeric and dimeric forms of photosystem II.

【 授权许可】

Unknown