Immunological studies on the D‐1 and D‐2 proteins of photosystem II preparations from the thermophilic cyanobacterium, Synechococcus sp

FEBS Letters

Satoh, Kazuhiko²  Oettmeier, Walter¹  Dostatni, Ralf¹  Johanningmeier, Udo¹ 

[1] Lehrstuhl Biochemie der Pflanzen, Ruhr- Universitat, D-4630 Bochum 1, FRG;Department of Pure and Applied Sciences, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153, Japan

关键词: D-1 protein;    D-2 protein;    Photosystem II;    Antibody;    (Synechococcus);    PS;    photosystem;    CBB;    Coomassie brilliant blue;   

DOI  :  10.1016/0014-5793(88)81344-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunoblotting of Synechococcus photosystem II preparations with site-directed antisera against the spinach D-2 protein and an antiserum against a large polypeptide segment of the spinach D-1 protein showed (i) that the 28 and 31 kDa polypeptides of the cyanobacterial photosystem II core complex are equivalent to the D-1 and D-2 proteins of higher plants and algae and (ii) that the β-octylglucoside treatment of the photosystem II preparation diminishes band intensities of the 28 and 31 kDa proteins in Coomassie blue-stained gels mainly by increasing the amounts of two proteins which migrate as a dimer of D₁ or D₂ or its aggregate, and of proteolytic digestion products.

【 授权许可】

Unknown