Complete exon‐intron organization of the mouse fibulin‐1 gene and its comparison with the human fibulin‐1 gene

FEBS Letters

Zhang, Rui-Zhu²  Chu, Mon-Li²  Kostka, Günter³  Pan, Te-Cheng²  Timpl, Rupert¹ 

[1] Department of Biochemistry and Molecular Pharmacology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA;Department of Dermatology and Cutaneous Biology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University, 233 South 10th Street, Philadelphia, PA 19107, USA;Max-Planck-Institut für Biochemie, D-82152 Martinsried, Germany

关键词: Fibulin-1;    Gene structure;    Extracellular matrix;    Alternative splicing;    EG module;    epidermal growth factor-like module;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(99)00024-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fibulin-1 is a 90 kDa calcium-binding protein present in the extracellular matrix and in the blood. Two major variants, C and D, differ in their C-termini as well as the ability to bind the basement membrane protein nidogen. Here we characterized genomic clones encoding the mouse fibulin-1 gene, which contains 18 exons spanning at least 75 kb of DNA. The two variants are generated by alternative splicing of exons in the 3′ end. By searching the database we identified most of the exons encoding the human fibulin-1 gene and showed that its exon-intron organization is similar to that of the mouse gene.

【 授权许可】

Unknown