Mouse cone arrestin gene characterization: promoter targets expression to cone photoreceptors

FEBS Letters

Ma, Bo²  Knox, Barry E¹  Craft, Cheryl M²  Zhu, Xuemei²  Babu, Sudha¹  Murage, Jaji² 

[1] Department of Biochemistry and Molecular Biology and Department of Ophthalmology, SUNY Upstate Medical University, Syracuse, NY 13210, USA;The Mary D. Allen Laboratory for Vision Research, Doheny Eye Institute, Department of Cell and Neurobiology, The Keck School of Medicine of the University of Southern California, 1333 San Pablo Street, BMT 401, Los Angeles, CA 90089-9112, USA

关键词: Cone arrestin;    Gene structure;    Alternative splicing;    Promoter activity;    Transgenic Xenopus laevis;    GPCR;    G-protein-coupled receptor;    CAR;    cone arrestin;    mCAR;    mouse cone arrestin;    CRX;    cone-rod homeobox;    ERK1 and 2;    extracellular signal-regulated kinases 1 and 2;    MAPKs;    mitogen-activated protein kinases;    JNK3;    c-Jun amino-terminal kinase 3;    EGFP;    enhanced green fluorescent protein;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    RACE;    rapid amplification of cDNA ends;    MTS;    minor transcription start site;   

DOI  :  10.1016/S0014-5793(02)03014-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cone arrestin (CAR) is a novel member of the arrestin superfamily expressed in retinal cone photoreceptors and the pineal gland. To understand the regulatory mechanisms controlling its cone- and pineal-specific expression, and to facilitate further functional studies using gene knockout approaches, we characterized the genomic organization and the 5′-flanking region of the mouse CAR (mCAR) gene. The mCAR gene is comprised of 17 exons and 16 introns, encoding five alternatively spliced transcripts. A 215-bp proximal promoter fragment containing a TATA box, an Sp1 site and four cone-rod homeobox-binding sites is sufficient to direct expression in cultured retinoblastoma cells and in cone photoreceptors and the pineal gland in transgenic Xenopus laevis.

【 授权许可】

Unknown