Characterisation of a novel Drosophila melanogaster testis specific PP1 inhibitor related to mammalian inhibitor‐2: identification of the site of interaction with PP1

FEBS Letters

Vergidou, Cornillia¹  Gaskell, Terri¹  Cohen, Patricia T.W.¹  Helps, Nicholas R.¹ 

[1] Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, UK

关键词: Protein phosphatase;    Inhibitor;    Two hybrid system;    cDNA sequence;    Drosophila melanogaster;    Testis;   

DOI  :  10.1016/S0014-5793(98)01286-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel Drosophila melanogaster protein, termed inhibitor-t, that bears 41% sequence similarity to human protein phosphatase inhibitor-2 has been identified using human protein phosphatase 1 (PP1) in the yeast two hybrid system. Inhibitor-t mRNA is detected in adult males, larvae and pupae and the 184 amino acid thermostable protein located only in testis. The gene for inhibitor-t maps to cytological location 86F1 on the third chromosome. Bacterially expressed inhibitor-t specifically inhibits both mammalian and D. melanogaster PP1 catalytic subunits with an IC₅₀ of approximately 200 nM. A motif -FEX₁X₂RK-, conserved between inhibitor-t, inhibitor-2 and its Saccharomyces cerevisiae homologue Glc8, is demonstrated to be required for binding to PP1.

【 授权许可】

Unknown