Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M

FEBS Letters

Chen, Yu Hua²  Alessi, Dario R.²  Chen, Mao Xiang²  Cohen, Patricia T.W.²  Shanahan, Catherine¹  Campbell, David G.²  Cohen, Philip² 

[1] Department of Medicine, Addenbrooke's Hospital, Cambridge, UK;Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland, UK

关键词: Smooth muscle;    Protein phosphatase;    cDNA sequence;    Myosin;    Ankyrin repeat;    Leucine zipper;   

DOI  :  10.1016/0014-5793(94)01231-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The structures of the M₁₁₀ and M₂₁ regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M₁₁₀ subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M₂₁ subunit from chicken gizzard contains a leucine zipper motif. The M₁₁₀ subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M₂₁ subunit.

【 授权许可】

Unknown