Cloning of the complete coding region for human protein phosphatase inhibitor 2 using the two hybrid system and expression of inhibitor 2 in E. coli

FEBS Letters

Elledge, Stephen J.¹  Cohen, Patricia T.W.²  Street, Alasdair J.²  Helps, Nicholas R.² 

[1] Howard Hughes Medical Institute, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA;Medical Research Council Protein Phosphorylotion Unit, Department of Biochemistry, The University, Dundee DD1 4HN, Scotland, UK

关键词: Inhibitor 2;    Protein phosphatase;    Two hybrid system;    cDNA sequence;    Human;   

DOI  :  10.1016/0014-5793(94)80179-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The yeast two hybrid system has been employed to identify cDNAs encoding proteins which interact with the γ₁, isoform of human protein phosphatase 1. Here we report the isolation of a cDNA encoding human protein phosphatase inhibitor 2. The deduced human sequence of 205 amino acids shows 92% identity to inhibitor 2 from rabbit. Human inhibitor 2 was expressed in E. coli and purified to homogeneity. The expressed human protein inhibited both native and bacterially expressed PP1, with the same K _i (1 nM) as inhibitor 2 purified from skeletal muscle. A gene or pseudogene for inhibitor 2 may be present near the major histocompatibility complex on chromosome 6.

【 授权许可】

Unknown