Drosophila melanogaster protein phosphatase inhibitor‐2: identification of a site important for PP1 inhibition

FEBS Letters

Cohen, Patricia T.W.¹  Helps, Nicholas R.¹ 

[1] Medical Research Council Protein Phosphorylation Unit, Department of Biochemistry, MSI/WTB Complex, University of Dundee, Dundee DD1 5EH, UK

关键词: Inhibitor-2;    Protein phosphatase;    cDNA sequence;    Drosophila melanogaster;   

DOI  :  10.1016/S0014-5793(99)01573-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A database search with human protein phosphatase inhibitor-2 (I-2) identified a Drosophila melanogaster cDNA that encoded a protein identical in length and sharing 39% amino acid identity (58% similarity) with human I-2. The mRNA encoding this protein is expressed in both sexes and throughout development, unlike Drosophila inhibitor-t. The bacterially expressed protein was a specific inhibitor of protein phosphatase 1 with an IC₅₀ of <1 nM, confirming that it is the Drosophila homologue of mammalian inhibitor-2. Mutation of Phe residues conserved in I-2 from lower and higher eukaryotes showed that Phe-33 is important for inhibition of PP1c.

【 授权许可】

Unknown