Free fatty acids regulate the uncoupling protein and alternative oxidase activities in plant mitochondria

FEBS Letters

Sluse, Francis E.³  Vercesi, Anibal E.²  Almeida, Andréa M.²  Jarmuszkiewicz, Wieslawa¹ 

[1] Department of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701 Poznan, Poland;Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, CP 6111, 13083-970 Campinas, SP Brazil;Laboratory of Bioenergetics, Center of Oxygen Biochemistry, Institute of Chemistry B6, University of Liège, Sart Tilman, B-4000 Liège, Belgium

关键词: Alternative oxidase;    Plant uncoupling mitochondrial protein;    Mitochondrion;    Linoleic acid;    Lycopersicon esculentum;    AOX;    alternative oxidase;    BHAM;    benzohydroxamate;    BSA;    bovine serum albumin;    CN;    cyanide;    DTT;    dithiothreitol;    FFA;    free fatty acids;    LA;    linoleic acid;    PUMP;    plant uncoupling mitochondrial protein;    Pyr;    pyruvate;   

DOI  :  10.1016/S0014-5793(98)00922-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two energy-dissipating systems, an alternative oxidase and an uncoupling protein, are known to exist in plant mitochondria. In tomato fruit mitochondria linoleic acid, a substrate for the uncoupling protein, inhibited the alternative oxidase-sustained respiration and decreased the ADP/O ratio to the same value regardless of the level of alternative oxidase activity. Experiments with varying concentrations of linoleic acid have shown that inhibition of the alternative oxidase is more sensitive to the linoleic acid concentration than the uncoupling protein activation. It can be proposed that these dissipating systems work sequentially during the life of the plant cell, since a high level of free fatty acid-induced uncoupling protein activity excludes alternative oxidase activity.

【 授权许可】

Unknown