Uncoupling protein and alternative oxidase of Dictyostelium discoideum: occurrence, properties and protein expression during vegetative life and starvation‐induced early development

FEBS Letters

Sluse, Francis E.²  Jarmuszkiewicz, Wiesawa¹  Behrendt, Maciej¹  Navet, Rachel² 

[1]Department of Bioenergetics, Institute of Molecular Biology and Biotechnology, Adam Mickiewicz University, Fredry 10, 61-701 Poznan, Poland

[2]Laboratory of Bioenergetics, Department of Life Sciences, Institute of Chemistry B6, University of Liège, Sart Tilman, B-4000 Liège, Belgium

关键词: Mitochondrion;    Alternative oxidase;    Uncoupling protein;    Development;    Dictyostelium discoideum;    AOX;    alternative oxidase;    BHAM;    benzohydroxamate;    BSA;    bovine serum albumin;    CAT;    carboxyatractyloside;    DdAOX;    alternative oxidase of Dictyostelium discoideum;    DdUCP;    uncoupling protein of Dictyostelium discoideum;    DTT;    dithiothreitol;    EGS;    ethylene glycol-bis-(succinimidylsuccinate);    FCCP;    carbonyl cyanide p-trifluoromethoxyphenylhydrazone;    FFA;    free fatty acids;    LA;    linoleic acid;    ROS;    reactive oxygen species;    state 3;    phosphorylating respiration in the presence of added ADP;    state 4;    resting respiration in the absence of added ADP;    UCP;    uncoupling protein;    ΔΨ;    mitochondrial transmembrane electrical potential;   

DOI  :  10.1016/S0014-5793(02)03734-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study we show that mitochondria of Dictyostelium discoideum contain both alternative oxidase (AOX) and uncoupling protein (UCP). AOX was stimulated by purine mononucleoside and was monomeric. UCP was stimulated by free fatty acids and was poorly sensitive to GTP. Both proteins collaborated in energy dissipation when activated together. AOX expression in free-living ameboid cells decreased strongly from exponential to stationary phase of growth but much less during starvation-induced aggregation. In contrast, UCP expression was constant in all conditions indicating permanent need. Our results suggest that AOX could play a role in cell differentiation, mainly by protecting prespore cells from programmed cell death.

【 授权许可】

Unknown