Mitochondria and cells produce reactive oxygen species in virtual anaerobiosis: relevance to ceramide‐induced apoptosis

FEBS Letters

Degli Esposti, Mauro¹  McLennan, Holly¹ 

[1] Department of Biochemistry and Molecular Biology, Monash University, Wellington Road, Clayton 3168, Victoria, Australia

关键词: Reactive oxygen species;    Mitochondrion;    Apoptosis;    Ceramide;    DCF;    dichlorofluorescein;    DCFDA;    dichlorodihydrofluorescein diacetate;    DPI;    diphenyleneiodonium;    MOA;    methoxy-acrylate;    Q;    ubiquinone;    ROS;    reactive oxygen species;    SMP;    submitochondrial particles;   

DOI  :  10.1016/S0014-5793(98)00688-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Observations of apoptosis in virtual anaerobiosis have raised doubts on the significance of reactive oxygen species in the cascade of events of programmed cell death. This work presents evidence that cells and mitochondrial preparations produce similar levels of hydrogen peroxide under either aerobic or virtually anaerobic conditions. These levels are relevant to the increased production of radicals induced by a ceramide analog that promotes apoptosis. This ceramide acts at center o of mitochondrial complex III.

【 授权许可】

Unknown