Peroxidative damage to cardiac mitochondria: cytochrome oxidase and cardiolipin alterations

FEBS Letters

Paradies, Giuseppe¹  Quagliariello, Ernesto¹  Petrosillo, Giuseppe¹  Ruggiero, Francesca Maria¹ 

[1] Department of Biochemistry and Molecular Biology and CNR Unit for the study of Mitochondria and Bioenergetics, University of Bari, Bari, Italy

关键词: Lipid peroxidation;    Cytochrome oxidase;    Cardiolipin;    Rat heart mitochondria;    t-BuOOH;    tert-butylhydroperoxide;    CL;    cardiolipin;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    Lipos;    liposomes;   

DOI  :  10.1016/S0014-5793(98)00161-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat heart mitochondrial membranes exposed to the free radicals generating system tert-butylhydroperoxide/Cu²⁺ undergo lipid peroxidation as evidenced by the accumulation of thyobarbituric acid reactive substances. Mitochondrial lipid peroxidation resulted in a marked loss of both cytochrome c oxidase activity and cardiolipin content. The alterations in the properties of cytochrome c oxidase were confined to a decrease in the maximal activity (V _max) with no change in the affinity (K _m) with respect to the substrate cytochrome c. Various lipid soluble antioxidants could prevent the lipid peroxidation reaction and the associated loss of cytochrome c oxidase activity. External added cardiolipin but no other phospholipids, nor peroxidized cardiolipin was able to prevent the loss of cytochrome oxidase activity induced by lipid peroxidation. These results establish a close correlation between oxidative damage to cardiolipin and alterations in the cytochrome oxidase activity and may prove useful in probing molecular mechanism of free radicals induced peroxidative damage of mitochondria which has been proposed to contribute to aging and to chronic degenerative diseases.

【 授权许可】

Unknown