Age‐dependent decline in the cytochrome c oxidase activity in rat heart mitochondria: role of cardiolipin

FEBS Letters

Paradies, Giuseppe¹  Quagliariello, Ernesto¹  Petrosillo, Giuseppe¹  Ruggiero, Francesca Maria¹ 

[1] Department of Biochemistry and Molecular Biology and CNR Unit for the Study of Mitochondria and Bioenergenetics University of Bari, Bari, Italy

关键词: Cytochrome oxidase;    Cardiolipin;    Aging;    Rat heart mitochondria;    CL;    cardiolipin;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    TMPD;    N;    N;    N′;    N′-tetramethyl-p-phenylene-diamine;    Lipo;    liposome(s);   

DOI  :  10.1016/S0014-5793(97)00264-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cardiolipin is a major mitochondrial membrane lipid and plays a pivotal role in mitochondrial function. We have recently suggested a possible involvement of this phospholipid in the age-linked decline of cytochrome c oxidase activity in rat heart mitochondria [G. Paradies et al. (1993) Arch. Gerontol. Geriatr. 16, 263–272]. The aim of this work was to test our earlier proposal. We have investigated whether addition of exogenous cardiolipin to mitochondria is able to reverse, in situ, the age-linked decrease in the cytochrome oxidase activity. The method of fusion of liposomes with mitochondria developed by Hackenbrock [Hackenbrock and Chazotte (1986) Methods Enzymol. 125, 35–45] was employed in order to enrich the mitochondria cardiolipin content. We demonstrate that the lower cytochrome c oxidase activity in heart mitochondria from aged rats can be fully restored to the level of young control rats by exogenously added cardiolipin. No restoration was obtained with other phospholipids or with peroxidized cardiolipin. Our data support a key role for cardiolipin in the age-linked decline of rat heart mitochondrial cytochrome c oxidase activity.

【 授权许可】

Unknown